¹⁵N D-CEST experiment
You can get the example files from the GitHub ChemEx page: regular and H/D solvent exchange studies.
The two examples demonstrate the application of the ¹⁵N D-CEST experiment
(dcest_15n). D-CEST experiment accelerates
data acquisition by using the DANTE scheme to perform multifrequency irradiation
at a desired effective B1 field. In D-CEST experiment, the saturation
element is replaced with DANTE pulses to perform multifrequency irradiation,
which results in dramatic savings in measurement time.1
An important application of D-CEST experiment is to measure H/D solvent exchange rates, the results should be more accurate than traditional CLEANEX experiment, especially for studying highly flexible biomolecules such as intrinsically disordered proteins.2
Use the kinetic model 2st_hd for H/D solvent exchange study. Besides, you
should provide the amount of D2O in the sample with the d2o key in
the section [conditions] of the experiment.toml file. The d2o key is
included in experiment files to differentiate datasets recorded with different
amounts of D2O. Note that the amount of D2O is a parameter
that can be fitted to account for potential errors during sample preparation.